New adult chat en vivo
Spinal cord injury (SCI) leads to irreversible neuronal loss and glial scar formation, which ultimately result in persistent neurological dysfunction.Cellular regeneration could be an ideal approach to replenish the lost cells and repair the damage.It also permitted imaging of single degenerating axons in adult brain and the first visualization of cleaved Caspase-3 in degenerating embryonic sensory axons in vivo, even single axons.
Our study indicates that resident astrocytes in the injured adult spinal cord can be manipulated to produce neurons by defined factors, raising the possibility of using these cells as a source for in situ repair of SCI.
Third, the transplantation procedure induces secondary injury to the spinal cord.
A relatively large quantity of cells is frequently needed for transplantation, which can result in unintended damage to preexisting functional neural circuits, fluid leak and the formation of cystic tissue at the injection site(s).
i DISCO is modeled on classical histology techniques, facilitating translation of section staining assays to intact tissues, as evidenced by compatibility with 28 antibodies to both endogenous antigens and transgenic reporters like GFP.
When applied to degenerating neurons, i DISCO revealed unexpected variability in number of apoptotic neurons within individual sensory ganglia despite tight control of total number in all ganglia.
These results clearly demonstrate that neural networks in the adult spinal cord exhibit structural plasticity, which provides a strong rationale for cell-based therapy.